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Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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요약
Automatic Translation

This protocol efficiently studies mammalian cell division in 3D collagen matrices by integrating synchronization of cell division, monitoring of division events in 3D matrices using live-cell imaging technique, time-resolved confocal reflection microscopy and quantitative imaging analysis.

Tags

Mammalian Cell Division 3D Matrices Quantitative Confocal Reflection Microscopy Cell Matrix Interactions Physiologically Relevant Efficient Approach Cell Biology Molecular Basis Normal Tissue Development Disease Tissue Development Transfection HEK-293 T-cells Cell Culture Medium Transfection Reagent Lentiviral Vector Plasmids Reduced Serum Medium
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