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Neuroscience

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JoVE Journal Neuroscience
GLUT4 タンパク質デコンボリューション顕微鏡を用いたマウス一次視床下部ニューロンにおける人身売買のライブ映像
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GLUT4 タンパク質デコンボリューション顕微鏡を用いたマウス一次視床下部ニューロンにおける人身売買のライブ映像

Article DOI: 10.3791/56409-v • 08:47 min • December 7th, 2017
December 7th, 2017 •
1,2,3, 4,5, 4,5,6
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

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このプロトコルは、タグ付きの観測リアルタイム緑色蛍光タンパク質 (GFP) のブドウ糖の運送者 4 (GLUT4) タンパク質インスリン刺激とインスリン-GLUT4 の CCR5 の生物学的役割の評価時に人身売買の手法を記述します。デコンボリューション顕微鏡を用いたシグナル伝達。

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神経生物学の問題 130、プライマリ マウス視床下部ニューロンの文化、ライブ画像記録、リポソームによる遺伝子導入、GLUT4 デコンボリューション顕微鏡
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