Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Andrew Ruba; Wangxi Luo; Weidong Yang

doi:10.3791/56475

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생물학

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Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

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JoVE 신문 생물학

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

8,232 Views

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07:53 min

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January 16, 2018

DOI:

10.3791/56475-v

DOI:

10.3791/56475-v

•

07:53 min

January 16, 2018

•

1 Views

Andrew Ruba¹, Wangxi Luo¹, Weidong Yang¹

¹Department of Biology,Temple University

내레이션 대본

Summary

Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.