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Journal / Genetics / Integrazione mirata CRISPR/Cas9-mediata In Vivo…
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Integrazione mirata CRISPR/Cas9-mediata In Vivo usando una strategia basata su unendo fine omologia-mediata

Article DOI: 10.3791/56844-v • 08:22 min • March 12th, 2018
March 12th, 2018 •
1,2, 1,2, 2,3,4, 1, 3, 1
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Il cluster regolarmente intervallate proteine brevi ripetizioni/CRISPR palindromi associato 9 (CRISPR/Cas9) sistema fornisce uno strumento promettente per l'ingegneria genetica e apre la possibilità di integrazione mirata dei transgeni. Descriviamo un fine omologia-mediata entrare (HMEJ)-base di strategia per DNA efficiente mirata integrazione in vivo e mirate terapie geniche utilizzando CRISPR/Cas9.

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Genetica problema 133 CRISPR/Cas9 integrazione mirata fine omologia-mediata entrare In Vivo embrione geneticamente topi iniezione idrodinamica
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