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Developmental Biology

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Klonen analyse van embryonale hematopoietische stamcellen precursoren met eencellige Index sorteren gecombineerd met Endothelial Niche co celkweek
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Klonen analyse van embryonale hematopoietische stamcellen precursoren met eencellige Index sorteren gecombineerd met Endothelial Niche co celkweek

Article DOI: 10.3791/56973-v • 09:32 min • May 8th, 2018
May 8th, 2018 •
1,2, 1, 1, 1, 1,2
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

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Hier presenteren we methodologie voor de klonale analyse van hematopoietische stamcellen precursoren tijdens lymfkliertest embryonale ontwikkeling. Wij combineren index sortering van afzonderlijke cellen uit de embryonale aorta-gonaden-mesonephros regio met endothelial co celkweek en transplantatie te karakteriseren de fenotypische eigenschappen en het engraftment potentieel van één hematopoietische precursoren.

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Ontwikkelingsbiologie kwestie 135 hematopoietische stamcellen (HSC) pre-HSC aorta-gonaden-mesonephros regio (AVA) embryonale Haematopoiese index Sorteren eencellige klonen analyse hematopoietische stamceltransplantatie
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