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Fluorescence de cellule unique résolution vivre Imaging of Drosophila horloges circadiennes dans cerveau larvaire Culture

Article DOI: 10.3791/57015-v • 07:05 min • January 19th, 2018
January 19th, 2018 •
1, 1
1Department of Genetics and Evolution, University of Geneva

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Le but du présent protocole est d’établir la culture ex vivo Drosophila cerveau larvaire optimisée pour surveiller des rythmes circadiens moléculaires avec imagerie Time-lapse à long terme de fluorescence. On discute également l’application de cette méthode pour les essais pharmacologiques.

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Neurosciences numéro 131 Neuroscience rythmes circadiens la communication neuronale imagerie Time-lapse ex vivo la culture cerveau journaliste fluorescent Drosophila
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