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1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
Chapters
- 00:04Title
- 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
- 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
- 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
- 08:59Conclusion
Células endoteliais de tumor são importantes determinantes do microambiente do tumor e o curso da doença. Aqui, um protocolo para o isolamento de células endoteliais puras e viáveis de carcinoma colorectal humana e cólon normal para ser usado em pesquisa de teste e patogênese da droga é descrito.
