JoVE Journal
Immunology and Infection
Immunology and Infection
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.
챕터
요약
Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.