Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Richard B. McCosh; Michael J. Kreisman; Kellie M. Breen

doi:10.3791/57894

타악기 황 호르몬 분 비의 평가 대 한 쥐에서 꼬리 팁 혈액 샘플링을 자주

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Neuroscience

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JoVE Journal Neuroscience

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

타악기 황 호르몬 분 비의 평가 대 한 쥐에서 꼬리 팁 혈액 샘플링을 자주

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05:58 min

July 4, 2018

DOI: 10.3791/57894-v

Richard B. McCosh¹, Michael J. Kreisman¹, Kellie M. Breen¹

¹Department of Reproductive Medicine,University of California, San Diego School of Medicine

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Overview

This study describes a tail-tip blood sampling protocol for the collection of blood samples in unrestrained mice, focusing on the measurement of pulsatile luteinizing hormone (LH) secretion. The method is designed to enable reliable assessments of LH pulsatile secretion, contributing to research in reproductive neuroendocrinology.

Key Study Components

Area of Science

Neuroendocrinology
Reproductive biology
Animal behavior

Background

Luteinizing hormone pulsatile secretion is critical in reproductive physiology.
Reliable blood sampling techniques are essential for accurately measuring hormonal fluctuations.
Existing methods may not adequately account for the effects of animal stress.
This protocol aims to minimize mouse stress during blood collection.

Purpose of Study

To develop a reliable tail-tip blood sampling protocol for unrestrained mice.
To assess LH pulsatile secretion in response to both acute and chronic treatments.
To improve the accuracy of neuroendocrinological research methods.

Methods Used

The protocol involves daily handling and habituation of mice prior to blood collection.
Adult female C57BL/6 mice were used, with blood collected from the tail tip for LH measurement.
Frequent handling sessions helped facilitate a stress-free environment.
Blood samples were collected over a set timeframe to establish hormonal baselines.

Main Results

The technique led to successful collection of LH levels in control animals.
Psychosocial stress from restraint significantly suppressed LH secretion in experimental groups.
Stress minimized reliable measurements of pulsatile LH secretion, highlighting the method's efficacy.
Environmental disturbances were found to affect hormonal measurements.

Conclusions

This study establishes a novel method for obtaining accurate hormonal measurements in mice.
The protocol allows researchers to explore LH regulation effectively in neuroendocrinology studies.
Understanding the impact of stress on hormonal secretion contributes to broader insights into reproductive physiology and animal behavior.

Frequently Asked Questions

What are the advantages of this blood sampling protocol?

This protocol minimizes stress by allowing mice to remain unrestrained, facilitating more reliable hormonal measurements.

How is the tail sample obtained?

Blood is collected by gently clipping the tail tip and stroking it to form a droplet, which is then sampled.

What is the biological model used in this study?

The study uses adult female C57BL/6 mice, which are well-characterized for neuroendocrinology research.

How does this method contribute to neuroendocrinology?

By providing reliable blood sampling under unrestrained conditions, researchers can better understand LH pulsatile secretion and its regulation.

What are key considerations when using this method?

Minimizing environmental disturbances is crucial, as noise and interruptions can impact hormonal measurements.

Can this method be adapted for other experiments?

Yes, the protocol can be adapted to assess other circulating factors, broadening its applicability in research.

여기 억제 쥐에서 꼬리 팁 혈액 샘플링 프로토콜 자주 샘플 컬렉션에 대 한 선물이. 이 메서드는 타악기 황 호르몬 분 비의 사정 패턴에 대 한 유용 하 고 다른 순환 요인의 분석에 대 한 적응 될 수 있습니다.

잦은 꼬리 끝 혈액 샘플링의 전반적인 목표는 깨어 있고 마취되지 않은 정상적인 행동을 하는 마우스에서 LH라고 하는 박동성 황체 형성 호르몬 분비를 측정하기 위한 전혈 샘플을 얻는 것입니다. 이 방법은 생쥐의 황체 형성 호르몬 박동 분비를 평가하기 위한 신뢰할 수 있는 프로토콜을 제공함으로써 재생 신경 내신학 분야의 주요 질문에 답하는 데 도움이 될 수 있습니다. 이 기술의 주요 장점은 잦은 취급과 습관화로 급성 또는 만성 실험 치료 후 강력하고 신뢰할 수 있는 황체 형성 호르몬 펄스 측정을 보장할 수 있다는 것입니다.

채혈 전 5주 동안 월요일부터 금요일까지 매일 쥐를 다루십시오. 마지막 2주 동안은 토요일과 일요일에도 생쥐를 처리하십시오. 생물 안전 캐비닛에서 마우스 취급 요법을 수행합니다.

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