전립선 암에 있는 Phosphoproteome를 조사 하기 위해 양적 라벨 무료 질량 분석으로 결합 된 Phosphopeptide 농축

14.1K 조회수

15회 인용됨

12:23 분

2018년 8월 2일

10.3791/57996-v

2018년 8월 2일

14.1K 조회수

Larry C. Cheng^*¹^,² , Zhen Li^*³ , Thomas G. Graeber⁴ , Nicholas A. Graham⁵ , Justin M. Drake¹^,²^,³^,⁶^,⁷

¹Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, ²Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, ³Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, ⁴Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, ⁵Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, ⁶Pharmacology, Rutgers Robert Wood Johnson Medical School, ⁷Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey

^* These authors contributed equally

이 프로토콜에는 전립선 암 세포 선 또는 단백질 질량 분석 기반 을 통해 phosphoproteome의 분석에 대 한 조직에서 phosphopeptides을 풍부 하 게 추출 하는 절차를 설명 합니다.

더 많은 동영상 탐색

전립선암 인산화 단백질체

단백질 소화

이산화티타늄 농축

액체 크로마토그래피 질량 분석법

포스포티로신 농축

포스포세린 트레오닌 농축

질량 분석 분석

Chapters in this video

0:04

Title

0:38

Harvesting Tissue

1:31

Lysate Digestion and Reverse Phase Extraction

4:12

Immunoprecipitation and Enrichment of pY Peptides and Prewashing of pY Beads

6:59

Titanium Dioxide Enrichment of pY Peptides and Desalting for MS Analyses

9:32

Results: Prostate Cancer Phosphopeptide Enrichment and Analysis by Mass Spectrometry

11:29

Conclusion