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Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Article DOI: 10.3791/58242-v • 07:22 min • November 20th, 2018
November 20th, 2018 •
*1, *1, 1,2, 1, 3, 4, 1,2
1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, 2State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, 3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, 4Shanghai Biozeron Biotechnology Co., Ltd
* These authors contributed equally

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요약
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Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.

Tags

Homo- And Heterografts Watermelon Bottle Gourd Cold-responsive MicroRNAs MiRNA Regulation Stress Response Efficient Method Reproducible Method Survival Rate Grafting Insight Cold Stress Response Long Distance MiRNA Transportation Visual Demonstration Advanced Skills Peat Soil Autoclave Distilled Water Sprouting Seeds
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