Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

Pierrette Courtois; Patricia Nabos; Romaric Nzoumbou-Boko; Christine E. Reix; Fr&#233;d&#233;ric-Antoine Dauchy; Sylvie Daulouede; Fr&#233;d&#233;ric Bringaud; Derrick  R. Robinson; Philippe Vincendeau

doi:10.3791/58415

음이온 교환기 (Diethylaminoethyl-셀 루 로스 열)에 의해 혈액에서 아프리카를 포함 한 세포 외 Trypan...

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Immunology and Infection
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JoVE Journal Immunology and Infection

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

음이온 교환기 (Diethylaminoethyl-셀 루 로스 열)에 의해 혈액에서 아프리카를 포함 한 세포 외 Trypanosomes의 정화

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14:26 min

April 6, 2019

DOI: 10.3791/58415-v

Pierrette Courtois^1,2, Patricia Nabos^1,2, Romaric Nzoumbou-Boko^1,2, Christine E. Reix³, Frédéric-Antoine Dauchy^1,2,4, Sylvie Daulouede^1,2, Frédéric Bringaud³, Derrick R. Robinson³, Philippe Vincendeau^1,2,4

¹Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, ²IRD UMR 177 INTERTRYP CIRAD, ³CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, ⁴Centre Hospitalier Université Bordeaux

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Overview

African trypanosomes are the causative agents of human and animal African trypanosomiasis, transmitted by tsetse flies. Accurate detection of these parasites in blood is crucial for diagnosis and treatment, necessitating concentration techniques for effective identification.

Key Study Components

Area of Science

Parasitology
Infectious Diseases
Diagnostic Methods

Background

African trypanosomiasis, also known as sleeping sickness, is a significant health concern in sub-Saharan Africa.
Serological tests can yield false positives and negatives, complicating diagnosis.
Concentration of trypanosomes in blood is essential for accurate detection.
Various techniques exist for concentrating these parasites.

Purpose of Study

To improve the diagnostic methods for detecting African trypanosomes in blood samples.
To provide purified parasites for further biological and medical research.
To enhance the understanding of trypanosomiasis and its impact on health.

Methods Used

Utilization of an anion-exchanger column for separating trypanosomes based on surface charge.
Application of concentration techniques to facilitate parasite detection.
Assessment of the method's effectiveness in providing purified samples.
Evaluation of the method's suitability for various biological investigations.

Main Results

The anion-exchanger column effectively separates trypanosomes from mammalian blood cells.
This method yields purified parasites suitable for immunological and biochemical studies.
Results indicate improved accuracy in diagnosing African trypanosomiasis.
The technique is applicable for various research fields, including molecular biology.

Conclusions

The developed method is a reliable diagnostic tool for African trypanosomiasis.
It provides a foundation for further research into the biology of trypanosomes.
Future studies can build on this method to enhance diagnostic accuracy and treatment strategies.

Frequently Asked Questions

What are African trypanosomes?

African trypanosomes are protist parasites that cause sleeping sickness in humans and animals, transmitted by tsetse flies.

Why is detecting trypanosomes in blood important?

Detection is crucial for accurate diagnosis, treatment, and monitoring of African trypanosomiasis.

What challenges exist in diagnosing African trypanosomiasis?

Serological tests can produce false positive and negative results, complicating the diagnosis.

What method is used for trypanosome separation?

An anion-exchanger column is used to separate trypanosomes based on their surface charge.

What are the applications of purified trypanosomes?

Purified trypanosomes can be used for immunological, biological, biochemical, pharmaceutical, and molecular biology investigations.

How does this method improve diagnostic accuracy?

The method effectively concentrates trypanosomes, leading to more reliable detection and diagnosis.

혈액에서 trypanosome 분리의이 방법은 포유류 혈액 세포 보다 덜 부정 되 고 그들의 표면 충전에 따라 다릅니다. 감염 된 혈액은 놓이고 음이온 교환기 열에 치료. 이 방법, 아프리카 trypanosomiasis에 대 한 가장 피팅 진단 면역학, 생물학, 생화학, 제약 및 분자 생물학 수사에 대 한 정화 기생충을 제공합니다.

아프리카 trypanosomes는 인간 아프리카 trypanosomiasis, 또는 잠자는 질병 및 동물 아프리카 trypanosomiasis에 책임 있습니다. 잠자는 질병을 일으키는 원인이 되는 trypanosomes는 사하라 사막 이남아프리카에 있는 많은 지리적 인 foci에 존재하는 tsete 비행 전송된 protist 기생충입니다. 기생충의 검출은 진단, 처리 및 후속을 위해 필수적입니다.

세로고는 거짓 긍정과 불행하게도, 거짓 부정적인 결과를 줄 수 있습니다. 혈액의 검출을 돕기 위해, trypanosomes는 집중되어야 합니다. 다양한 기생충 농도 기술이 사용되었습니다.

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