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Isolatie van CD4+ T-cellen en analyse van de circulerende T-folliculaire Helper (cTfh) cel Subsets van 6-kleur met behulp van perifere bloed stroom Cytometry

Article DOI: 10.3791/58431-v • 07:39 min • January 7th, 2019
January 7th, 2019 •
1, 1, 2, 1, 1
1Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute, University of Leicester, 2MRC Toxicology Unit, University of Leicester

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Hier, een protocol voor de isolatie en de karakterisering van CD4+ T-cel subsets van menselijke perifeer bloed wordt beschreven. CD4 gezuiverd+ T-cellen worden geanalyseerd door stroom cytometry om te bepalen van de verhoudingen van T-folliculaire helper cel subsets.

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Biologie kwestie 143 CD4 T-cel stroom cytometry perifeer bloed T-folliculaire helper cel circulerende T-folliculaire helper cel cel subsets
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