JoVE Journal
Biochemistry
Biochemistry
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.