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Journal / Biology / Isolatie van muizen epidermale keratinocyten en…
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Isolatie van muizen epidermale keratinocyten en hun in vitro Clonogene cultuur

Article DOI: 10.3791/58701-v • 06:16 min • August 10th, 2019
August 10th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hormel Institute, University of Minnesota

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Het doel van dit protocol is het isoleren van epidermale keratinocyten uit de rughuid van volwassen muizen voor een verscheidenheid aan downstreamtoepassingen zoals moleculaire biologie, biochemie, fluorescentie geactiveerde celsortering en primair in vitro gebruik (bijv. clonogene keratinocyten).

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Biologie afgifte 150 epidermale keratinocyten celkweek volwassen muis epidermis klonale cultuur Keratinocyt stamcellen 3T3 fibroblast
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