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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Article DOI: 10.3791/58759-v • 08:31 min • November 26th, 2018
November 26th, 2018 •
1, 1, 1, 1, 1
1Institute of Biochemistry and Cell Biology, OVGU University of Magdeburg

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요약
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Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.

Tags

Shear Flow-induced Migration Murine Marginal Zone B Cells In Vitro Analysis Immune Cell Migration Cytoskeleton Shear Forces Integrin Ligands Chemokines Leukocytes Migration Assay Beginners Commercially Available Equipment Reagents Free Software Insight Into Marginal Zone B Cells Activated T Cells ICAM-1 Flow Slide Incubate Overnight Wash Chamber Blocking Buffer Migration Buffer
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