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Developmental Biology

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JoVE Journal Developmental Biology
Effektiv differentiering av humana pluripotenta stamceller till leverceller
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Effektiv differentiering av humana pluripotenta stamceller till leverceller

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Detta protokoll specificerar en monolayer, serum fri metod för att effektivt generera hepatocyte-liknande celler från humana pluripotenta stamceller (hPSCs) i 18 dagar. Detta innebär sex steg som hpscs sekventiellt differentieras till mellanliggande cell-typer såsom primitiva strimma, definitiva endoderm, posterior framtarmen och lever bud stamceller innan de bildar hepatocyte-liknande celler.

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Utvecklingsbiologi utgåva 148 Human pluripotenta stamcell effektiv differentiering endoderm lever progenitor hepatocyte
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