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Immunology and Infection

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A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Article DOI: 10.3791/59033-v • 08:46 min • November 12th, 2019
November 12th, 2019 •
1, 2, 2, 2, 2, 2, 2, 2, 2, 1,3, 1, 1, 1, 2
1ACEA Biosciences, Inc, 2ProMab Biotechnologies. Inc, 3ACEA Therapeutics

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요약
Automatic Translation

We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.

Tags

Real-time Potency Assay Chimeric Antigen Receptor T Cells Solid Cancer Cells Hematological Cancer Cells XCELLigence Real-time Cell Analysis Label-free Conditions Multiple Days Target Cancer Cell Killing Screening Constructs Effector Cell Types Combination Therapies HEK293FT Cells DMEM Culture Medium Lentiviral Vector Plasmid DNA Lentiviral Packaging Mix Transfection Reagent Supernatant Centrifugation
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