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Immunology and Infection

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JoVE Journal Immunology and Infection
A Real-Time potens analyse for chimeric antigen reseptor T celler målretting solid og hematologiske kreftceller
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A Real-Time potens analyse for chimeric antigen reseptor T celler målretting solid og hematologiske kreftceller

Article DOI: 10.3791/59033-v • 08:46 min • November 12th, 2019
November 12th, 2019 •
1, 2, 2, 2, 2, 2, 2, 2, 2, 1,3, 1, 1, 1, 2
1ACEA Biosciences, Inc, 2ProMab Biotechnologies. Inc, 3ACEA Therapeutics

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Vi beskriver en kvantitativ Real-Time in vitro cytolyse analysen system for å evaluere styrken av chimeric antigen reseptor T-celler rettet mot flytende og solide tumorceller. Denne protokollen kan utvides til å vurdere andre immun Effektor celler, samt kombinasjonsbehandlinger.

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Immunologi og infeksjon chimeric antigen reseptor adoptiv celle terapi lentiviral genoverføring T-celler kreft immunterapi RTCA in vitro funksjonell analysen cytotoksisitet potens
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