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JoVE Journal Genetics
ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T
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ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Nous présentons ici un protocole pour effectuer un test pour la transposase accessible chromatine séquençage (ATAC-seq) sur activé CD4 + lymphocytes humains. Le protocole a été modifié pour réduire au minimum la contamination mitochondrial DNA lectures de 50 % à 3 % grâce à l’introduction d’une nouvelle mémoire tampon de lyse.

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Génétique numéro 145 épigénomique ATAC-seq Tn5 les lymphocytes CD4 + noyaux contamination de l’ADN mitochondriale
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