레브 면역 침전 및 질량 분광법을 통한 HIV-1 복제 중 핵균 요인 식별
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09:38 min
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June 26, 2019
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Summary
Automatically generated
여기에서 우리는 질량 분광법을 위한 HIV-1 복제의 존재에 있는 Rev 면역 침전을 기술합니다. 기재된 방법은 HIV-1 감염 주기에 관여하는 뉴클레올러 인자의 식별을 위해 사용될 수 있으며, 연구되지 않은 경로의 특성화를 위한 다른 질병 모델에 적용가능하다.
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Cite this Article
Arizala, J. A. C., Chomchan, P., Li, H., Moore, R., Ge, H., Ouellet, D. L., Rossi, J. J. Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry. J. Vis. Exp. (148), e59329, doi:10.3791/59329 (2019).
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