JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:44Oligonucleotide Library Design and Preparation and Bacteria Culture
- 02:07Nuclease Activity Assay Setup
- 03:05Measurement Setup
- 04:32Data Analysis
- 05:12Results: Representative Kinetic Screening
- 06:23Conclusion
Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.
