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Developmental Biology

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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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요약
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This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape changes.

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Dissection Culture Analysis Primary Cranial Neural Crest Cells Mouse Study Neural Crest Cell Delamination Migration Birth Defects Neural Plate Cell Behaviors Mutant Genes Pharmaceutical Treatment Health Screening Drug Treatment Environmental Changes Embryonic Development Heart Migration Gut Migration Metastatic Tumor Cells Anatomy Of The Embryo Microdissection Embryonic Day 8.5
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