An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Talita  Miguel Marin; Nathalia  de Carvalho Indolfo; Silvana  Aparecida Rocco; Murilo de Carvalho; Marilia  Meira Dias; Graziele Izalina Vasconcelos Bento; Leandro Oliveira Bortot; Desir&#233;e Cigaran Schuck; M&#225;rcio Lorencini; Eduardo Pagani

doi:10.3791/60184

Journal

/

화학

/

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

JoVE 신문
화학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 화학

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

7,708 Views

•

08:59 min

•

December 03, 2020

DOI:

10.3791/60184-v

DOI:

10.3791/60184-v

•

08:59 min

December 03, 2020

•

8 Views

Talita Miguel Marin¹, Nathalia de Carvalho Indolfo⁴, Silvana Aparecida Rocco¹, Murilo de Carvalho^1,2, Marilia Meira Dias¹, Graziele Izalina Vasconcelos Bento¹, Leandro Oliveira Bortot¹, Desirée Cigaran Schuck³, Márcio Lorencini³, Eduardo Pagani¹

¹Brazilian Biosciences National Laboratory (LNBio),Brazilian Center for Research in Energy and Materials (CNPEM), ²Brazilian Synchrotron Light Laboratory (LNLS),Brazilian Center for Research in Energy and Materials (CNPEM), ³Grupo Boticário, ⁴Institute of Biology,University of Campinas

Summary

We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.