Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Piyan Zhang; Irina  V. Kovtun

doi:10.3791/60646

Journal

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암 연구학

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Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

JoVE 신문
암 연구학
Author Produced

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JoVE 신문 암 연구학

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

7,232 Views

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10:27 min

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July 25, 2020

DOI:

10.3791/60646-v

DOI:

10.3791/60646-v

•

10:27 min

July 25, 2020

•

7195 Views

Piyan Zhang¹, Irina V. Kovtun^1,2

¹Center for Individualized Medicine,Mayo Clinic ²Department of Molecular Pharmacology and Experimental Therapeutics,Mayo Clinic

Summary

Here we present a protocol to test the efficacy of targeted therapies selected based on the genomic makeup of a tumor. The protocol describes identification and validation of structural DNA rearrangements, engraftment of patients’ tumors into mice and testing responses to corresponding drugs.