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Modello di trapianto di Murine (ISWAT) del tessuto adiposo sottocutaneo inguinale (ISWAT)

Article DOI: 10.3791/60679-v • 10:14 min • February 16th, 2020
February 16th, 2020 •
*1, *1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 1, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences, Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University
* These authors contributed equally

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In questo protocollo, viene descritto un metodo di isolamento murine ilet e trapianto nel tessuto adiposo bianco sottocutaneo inguinale. Le isole di murine singeniche isolate vengono trapiantate in un recipiente murino utilizzando un idrogel di membrana sotterranea. Viene monitorato il livello di glucosio nel sangue dei destinatari e viene eseguita l'analisi istologia degli innesti di islet.

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Medicina Numero 156 trapianto di isole isole murine T1DM sito di trapianto ISWAT idrogel
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