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Immunology and Infection

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Verduidelijken en beeldvorming Candida albicans Biofilms

Article DOI: 10.3791/60718-v • 11:09 min • March 6th, 2020
March 6th, 2020 •
1, 1, 1, 2, 1, 1
1Department of Biological Sciences, Carnegie Mellon University, 2Department of Pharmacology, Second Military Medical University

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Om de interne kenmerken van Candida albicans biofilms te bekijken en te kwantificeren, bereiden we vaste intacte exemplaren voor die worden verduidelijkt door refractieve indexmatching. Vervolgens kan optische sectioning microscopie worden gebruikt om driedimensionale beeldgegevens te verkrijgen, hoewel de volledige dikte van de biofilm.

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Immunologie en infectie kwestie 157 Candida albicans biofilm refractive index matching methyl salicylaat verduidelijking confocale microscopie
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