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Immunology and Infection

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JoVE Journal Immunology and Infection
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Article DOI: 10.3791/60806-v • 07:50 min • March 30th, 2020
March 30th, 2020 •
1, 1, 2, 1,3
1Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine, Northwestern University, 2Department of Pathology, University of Illinois at Chicago, 3Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University

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요약
Automatic Translation

We describe a rapid staining method to perform multispectral imaging on frozen tissues.

Tags

Rapid Method Multispectral Fluorescence Imaging Frozen Tissue Sections Detect Biomarkers Co-localize Biomarkers Cryosection Paraffin Sections Experimental Staining Method Staining Time Reduced Any Laboratory Dinesh Jaishankar Postdoc Immunotherapy Assessment Core Facilities Manager Antibodies Selection Excitation Filter Sets Emission Filter Sets Semi-automated Imaging System Fluorophore Conjugated Primary Antibodies Minimal Spectral Overlap Multiplex Staining Cocktail Of Antibodies Optimal Dilutions Tissue Sections Single Marker Staining Control Unstained Slide Incubation At Room Temperature Wash Slides With PBS Counter Stain With DAPI
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