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JoVE Journal Developmental Biology
Fluorescerende immunolocalisering af Arabinogalactan proteiner og pektiner i cellevæggen af plantevæv
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Fluorescerende immunolocalisering af Arabinogalactan proteiner og pektiner i cellevæggen af plantevæv

Article DOI: 10.3791/61034-v • 10:14 min • February 27th, 2021
February 27th, 2021 •
1,2, 3, 1,2
1Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze, Universitá degli Studi di Milano

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Denne protokol beskriver i detaljer, hvordan plantematerialet til immunolocalisering af Arabinogalactan proteiner og pektiner er fastgjort, indlejret i en hydrofil akrylharpiks, sektionsopdelt og monteret på glasrutsjebaner. Vi viser cellevæg relaterede epitoper vil blive opdaget med specifikke antistoffer.

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Udviklingsbiologi Problem 168 Arabinogalactan proteiner cellevægge Fluorescerende immunolocalization Histology immunocytochemistry LR-hvid harpiksindlejring pektiner Plantecelle Plantevæv
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