Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Click-Chemistry Based Fluorometric Assay for…
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Biochemistry
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae (Video) | JoVE
Journal (Biochemistry)
TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae (Video) | JoVE
Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE
Journal (Biochemistry)
Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE
 

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Article DOI: 10.3791/61146-v • 07:37 min • May 13th, 2020
May 13th, 2020 •
1, 1, 1
1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

챕터

요약
Automatic Translation

Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.

Tags

Click-Chemistry Fluorometric Assay Apolipoprotein N-acyltransferase Enzyme Characterization High-Throughput Screening HDS Format Inhibitors Protocol Sensitive Assay Screening Purposes Potential Novel Antibiotics Mixing Vortexing Sonication Nonhomogeneous Lipid Detergent Containing Suspensions Reagent Mixture Alkyne POPE FSL-1-biotin LNT Reaction Buffer Triplicate Reagent Mixtures Sonication Ultrasonic Water Bath Incubation Active LNT Inactive LNT Concentration Thermal Mixer Heated Lid Stock Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter