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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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요약
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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

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In Vivo CRISPR/Cas9 Screening Gene Function Mouse Skin Oral Cavity Tumor Suppressive Functions Head And Neck Cancer Knockout Mouse Model Targeted CRISPR Library Protocol Cancer Types Anesthetized Animal Surgery Stage Nosecone Anesthesia Tube Isoflurane Oxygen E9.5 Pregnancy Confirmation Hair Removal Cream Abdomen Ultrasound Probe Gel Embryonic Day E9.5 Subcutaneous Analgesic Vertical Incision Peritoneum
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