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In Vitro Utvärdering av Oncogenic Transformation i mänskliga mammary Epitelial celler

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Detta protokoll ger experimentella in vitro-verktyg för att utvärdera omvandlingen av mänskliga bröstceller. Detaljerade steg för att följa upp cell spridningshastighet, anchorage-oberoende tillväxt kapacitet och fördelning av cellen härstamningar i 3D kulturer med källare membran matris beskrivs.

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Cancerforskning cellulär transformation tillväxtkurva förankringsanalys 3D-cellkultur mänskliga bröstepiteceller tumorigenesis
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