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Biochemistry

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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Article DOI: 10.3791/61823-v • 05:56 min • November 12th, 2020
November 12th, 2020 •
*1, *2, *1, 3, 4, 2, 1, 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ
* These authors contributed equally

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요약
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This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

Tags

Spot Variation Fluorescence Correlation Spectroscopy Plasma Membrane Living Cells Lateral Organization Innovative Techniques Time Resolution Spatial Resolution Non-invasive Physiological Conditions Iris Diaphragms Laser Power Alignment Detection Path Rhodamine 6G Solution Correlator Software Noise Level Waste Size Count Rate Molecule Per Second Spot Size Calibration
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