사카로미세스 세레비시아스 스플리케소터 서브콤플렉스의 과도성 행위자 검출을 위한 그라픽스의 활용
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November 09, 2020
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Summary
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여기서는 크로스링커가 있는 글리세롤 그라데이션 원심분리인 그라픽스(Gradient Fixation)의 활용을 설명하여 쌍향적인 복합체에 일시적으로 결합하는 접합 요인 간의 상호 작용을 식별합니다.
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Cite this Article
Carvalho, F. A., Barros, M. R. A., Girotto, T. P. F., Perona, M. G., Oliveira, C. C. Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes. J. Vis. Exp. (165), e61994, doi:10.3791/61994 (2020).
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