원소 이미징을 위한 습지 뿌리와 류스피어를 보존하는 방법
3,392 Views
•
06:29 min
•
February 15, 2021
DOI:
Summary
Automatically generated
우리는쌀(Oryza sativa L.)을 모형 종으로 사용하여 습지 환경에서 온전한 뿌리와 주변 의체 토양을 시료, 보존 및 섹션화하는 프로토콜을 설명합니다. 일단 보존되면, 시료는 싱크로트론 X선 형광(XRF) 화학적 분석 영상과 같은 원소 이미징 기술을 사용하여 분석될 수 있다.
Related Videos
09:55
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Related Videos
26775 Views
11:37
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
Related Videos
16172 Views
11:57
Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions
Related Videos
18472 Views
10:31
Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Related Videos
54652 Views
07:59
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
Related Videos
10111 Views
07:45
An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
Related Videos
15806 Views
09:17
Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores
Related Videos
12066 Views
12:03
Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil
Related Videos
6095 Views
10:22
MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
Related Videos
5453 Views
08:28
Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays
Related Videos
2743 Views
Read Article
Cite this Article
Seyfferth, A. L., Limmer, M. A., Tappero, R. A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging. J. Vis. Exp. (168), e62227, doi:10.3791/62227 (2021).
Copy