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Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Article DOI: 10.3791/62352-v • 07:17 min • April 26th, 2021
April 26th, 2021 •
1, 2, 3, 4, 4
1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

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We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

Tags

Motility Patterns Stentor Oral Apparatus Regeneration Cell Tracking Model Organism Unicellular Regeneration Functional Regeneration Assay Recovery Of Loss Function Cell Types Imaging Chamber Silicone Spacers Leakage Test Sample Chambers Construction Motility Patterns Extraction
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