Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Practical Aspects of Sample Preparation and Setup …
JoVE Journal
Biochemistry

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biochemistry
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Journal (Biochemistry)
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
Journal (Biochemistry)
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
 

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Article DOI: 10.3791/62470-v • 08:17 min • July 9th, 2021
July 9th, 2021 •
1, 1, 1
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet

챕터

요약
Automatic Translation

We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R1ρ relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

Tags

RNA Sample Preparation NMR Setup Proton R1rho Relaxation Dispersion Confirmational Exchange Shifted Base-pairing Isotopic Labeling In Vitro Transcription Cleavage Reaction Denaturing PAGE Gel Incomplete Cleavage Microwave Heating Reanneal RNA Inorganic Pyrophosphatase RNase H
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter