Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Preparation of Human Tissues Embedded in Optimal…
JoVE Journal
Cancer Research

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Cancer Research
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Journal (Cancer Research)
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Journal (Cancer Research)
Using the E1A Minigene Tool to Study mRNA Splicing Changes
 

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Article DOI: 10.3791/62552-v • 09:09 min • April 27th, 2021
April 27th, 2021 •
1, 2, 1,3
1Department of Biology, Virginia Commonwealth University, 2VCU Lipidomics/Metabolomics Shared Resource, Virginia Commonwealth University School of Medicine, 3Massey Cancer Center

챕터

요약
Automatic Translation

Sphingolipids are bioactive metabolites with well-established roles in human disease. Characterizing alterations in tissues with mass-spectrometry can reveal roles in disease etiology or identify therapeutic targets. However, the OCT-compound used for cryopreservation in biorepositories interferes with mass-spectrometry. We outline methods to analyze sphingolipids in human tissues embedded in OCT with LC-ESI-MS/MS.

Tags

Human Tissues Optimal Cutting Temperature Compound (OCT) Mass Spectrometry Analysis Cryo-preservation Agent Tandem Liquid Chromatography Mass Spectrometry Sphingolipids Tissue Drying Wicks Tissue Washing Tissue Weighing Surgical Scissors Methanol Laboratory Grade Tissue Powder-free Gloves Pipette Tip Ultrapure Deionized Water Human Lung Tissue Incubate Vortex
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter