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Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
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Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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요약
Automatic Translation

This paper lists the steps required to seed neuroblastoma cell lines on previously described three-dimensional collagen-based scaffolds, maintain cell growth for a predetermined timeframe, and retrieve scaffolds for several cell growth and cell behavior analyses and downstream applications, adaptable to satisfy a range of experimental aims.

Tags

Three-dimensional In Vitro Biomimetic Model Neuroblastoma Collagen-based Scaffolds Pediatric Cancer Tumor Tissue Extracellular Matrix (ECM) Disease Progression Patient Prognosis Therapeutic Response Neuroblastoma Cell Lines Nanohydroxyapatite (nHA) Glycosaminoglycans (GAGs) Bone And Bone Marrow Metastatic Sites Porous Structure Cell Attachment Proliferation Migration Cell Clusters Therapeutics In Vivo Situation Scaffold-based Culture System Two-dimensional (2D) Cell Culture DNA Quantification Cell Viability
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