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Immunology and Infection
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Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

Article DOI: 10.3791/63063-v • 04:06 min • February 12th, 2022
February 12th, 2022 •
1, 1
1College of Veterinary Medicine, Department of Population Health and Pathobiology, North Carolina State University

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요약
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This protocol shows an accurate and objective approach to visualize viral titrations using crystal violet, by comparing it with optical microscopy and immunocytochemical staining.

Tags

Crystal Violet Visual Cytopathic Effect Viral Titration TCID50 Assays Virus Stock Staining 96-well Plate Biosafety Level 2 Laboratory Cell Line Dilutions Vortex Media PBS Inoculum Incubation PFA Fixation
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