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Biology
Gryllus bimaculatus의 실험실 문화 유지, 곤충 농업 및 무척추 동물 생리학을위한 다목적 ort...
Gryllus bimaculatus의 실험실 문화 유지, 곤충 농업 및 무척추 동물 생리학을위한 다목적 ort...
JoVE Journal
Biology
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JoVE Journal Biology
Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

Gryllus bimaculatus의 실험실 문화 유지, 곤충 농업 및 무척추 동물 생리학을위한 다목적 orthopteran 모델

Full Text
3,896 Views
08:30 min
June 8, 2022

DOI: 10.3791/63277-v

Martin K. Ventura1, Valerie J. Stull2, Susan M. Paskewitz1

1Department of Entomology,University of Wisconsin-Madison, 2Global Health Institute,University of Wisconsin-Madison

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Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This paper describes standardized methods for the long-term rearing of Gryllus bimaculatus, an important model organism and food source. The approaches outlined are derived from practices common in the North American cricket farming industry.

Key Study Components

Research Area

  • Laboratory rearing techniques
  • Edible cricket farming
  • Alternative model organisms

Background

  • Gryllus bimaculatus is increasingly used for both human and animal nutrition.
  • Standardized rearing practices ensure consistency in laboratory studies.
  • Techniques mimic real-world farming conditions.

Methods Used

  • Detailed methods for preparing substrates and managing environmental conditions
  • Gryllus bimaculatus as the primary organism
  • Laboratory-scale cricket farming operations

Main Results

  • Establishment of a reliable rearing system
  • Successful incubation and hatching of eggs
  • Monitoring and maintaining health indicators

Conclusions

  • This study demonstrates effective techniques for rearing crickets in a laboratory setting.
  • The findings are significant for advancing research in alternative protein sources and model organism studies.

Frequently Asked Questions

What is Gryllus bimaculatus used for?
Gryllus bimaculatus is used as a food source for both humans and animals, and it serves as an alternative model organism in research.
Why is standardization important in laboratory rearing?
Standardization helps ensure consistent results and facilitates replication of experiments across different studies.
What methods are used to maintain hydration in the substrate?
A five-to-one ratio of water to dry coconut coir is used to maintain optimal hydration conditions for rearing.
How does this research impact cricket farming?
The techniques can help improve cricket farming operations by providing a laboratory benchmark for best practices in rearing.
What conditions are required for incubation of crickets?
Crickets require an incubator set to 27 degrees Celsius and humidity levels around 60%, with a 12-hour light/dark photoperiod.
How often should feed be replaced during rearing?
Feed should be replaced every two days to prevent mold growth and ensure optimal nutrition.
What indicators are monitored for the health of the crickets?
Key indicators include growth rates, feeding behavior, and health of the environment, including mold presence on feed.

이 논문은 식용 귀뚜라미, Gryllus bimaculatus의 실험실 배양을 장기간 양육하기위한 밀도, 사료 가용성, 수화 공급원 및 환경 통제와 같은 중요한 요소를 표준화하는 기본 방법을 설명합니다.

이러한 기술은 동물 사료 및 인간 사료 및 점점 더 중요한 대체 모델 유기체를 위해 양식되는 주요 미니 가축 종 인 Gryllus bimaculatus를 기르기위한 실용적이고 입증 된 방법을 묘사합니다. Gryllus bimaculatus의 문화를위한 이러한 실험실 방법은 북미 크리켓 농업 산업 전반에 걸쳐 공통적 인 기술에서 파생되었으며 귀뚜라미 농업 운영의 실험실 규모의 팩시밀리를 나타냅니다. 시작하려면 깨끗한 용기를 저울에 꽂고 마른 코코넛 열매 덩어리를 인간의 주먹 크기로 측정하십시오.

그런 다음 coir를 밀봉 가능한 깨끗한 용기에 넣어 원래 부피의 최대 여섯 배까지 확장 할 수 있습니다. 깨끗한 손으로 더 큰 블록에서 제거 된 조각에서 coir 덩어리를 부드럽게 분해하십시오. 다음으로, 250 밀리리터 눈금 실린더를 사용하여, 탈이온수의 정확한 부피를 측정하여 한 부분 건조 코아에 5 부의 물의 질량 기준으로 다섯 대 일 비율을 달성하고, 측정 된 탈이온수를 천천히 첨가하여 모든 누아 입자를 고르게 수화시킵니다.

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