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Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can be a potential source of MSCs that differentiate into insulin-producing cells (IPCs). In this protocol, we provide detailed steps for the isolation and characterization of rat epididymal Ad-MSCs, followed by a simple, short protocol for the generation of IPCs from the same rat Ad-MSCs.

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Isolation Rat Adipose Tissue Mesenchymal Stem Cells Differentiation Insulin-producing Cells Protocol Red Adipose Tissue Dried Simple Methods Genetics Sources Bone Marrow Perinatal Tissue Cultural Characteristics Multi Potent Disease Treatment Lipoaspirates Yield Autologous Transplantation Allogenic Transplantation Isolation Protocol Characterization Protocol Epididymal Fat Collagenous Digestion Vascular Fraction Plastic Adherence CD Markers
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