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JoVE Journal Cancer Research
Een labelvrije segmentatiebenadering voor intravitale beeldvorming van de micro-omgeving van borsttumoren
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Een labelvrije segmentatiebenadering voor intravitale beeldvorming van de micro-omgeving van borsttumoren

Article DOI: 10.3791/63413-v • 10:39 min • May 24th, 2022
May 24th, 2022 •
1, 1, 1,2, 1, 1,3
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Pathology, University of Wisconsin-Madison, 3Carbone Cancer Center, University of Wisconsin-Madison

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De hier beschreven intravitale beeldvormingsmethode maakt gebruik van collageen tweede harmonische generatie en endogene fluorescentie van de metabole co-factor NAD (P) H om een niet-invasieve tumormicro-omgeving te segmenteren in tumor-, stromale en vasculaire compartimenten voor diepgaande analyse van 4D intravitale beelden.

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