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Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

Article DOI: 10.3791/63779-v • 08:14 min • December 9th, 2022
December 9th, 2022 •
1, 1, 2, 2, 1
1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

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The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

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Extraction Non-protein Amino Acids Cyanobacteria Liquid Chromatography-tandem Mass Spectrometry Analysis BMA AEG 2 4-DAB Error Minimization Consistent Results Toxicity Research Health Effects Centrifugation Biological Waste Sealing Film Freeze Drying
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