Sample Preparation for Rapid Lipid Analysis in <em>Drosophila</em> Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Yuki X. Chen; Kelly Veerasammy; Jun Yin; Tenzin Choetso; Tiffany Zhong; Muniyat A. Choudhury; Cory Weng; Ethan Xu; Mayan A. Hein; Rinat Abzalimov; Ye He

doi:10.3791/63930

매트릭스 보조 레이저 탈착/이온화 질량 분광법 이미징 을 사용한 초파리 뇌의 신속한 지질 분...

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Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

매트릭스 보조 레이저 탈착/이온화 질량 분광법 이미징 을 사용한 초파리 뇌의 신속한 지질 분석을 위한 샘플 준비

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09:00 min

July 14, 2022

DOI: 10.3791/63930-v

Yuki X. Chen*^1,5,6, Kelly Veerasammy*^1,2, Jun Yin*³, Tenzin Choetso^1,4, Tiffany Zhong⁷, Muniyat A. Choudhury^1,4,5, Cory Weng^1,4,5, Ethan Xu⁸, Mayan A. Hein^9,10, Rinat Abzalimov², Ye He¹

¹Advanced Science Research Center, Neuroscience Initiative,the City University of New York, Graduate Center New York, ²Advanced Science Research Center, Structural Biology Initiative,the City University of New York, Graduate Center, ³National Institute of Neurological Disorders and Stroke,National Institutes of Health, ⁴The City College of New York,CUNY, ⁵Macaulay Honors College,CUNY, ⁶Graduate School of Public Health and Health Policy,The City University of New York, ⁷Princeton University, ⁸Ardrey Kell High School, ⁹The Borough of Manhattan Community College,CUNY, ¹⁰Gallatin School of Individualized Study,New York University

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Overview

This protocol offers thorough guidance on preparing lipid and metabolite samples from small tissues, such as the Drosophila brain, employing matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. The method allows for the visualization of lipid distribution without significant sample modification.

Key Study Components

Research Area

Metabolite analysis
Lipid distribution
Mass spectrometry imaging

Background

MALDI mass spectrometry imaging is critical for lipid detection in situ.
Minimizing sample preparation time is essential to avoid contamination.
The methodology supports simultaneous analysis of large sample populations.

Methods Used

Sample preparation involves optimizing the cutting temperature and embedding tissues in optimal cutting temperature (OCT) compound.
Drosophila melanogaster is utilized as a model organism.
The MALDI imaging technology enables detailed lipid abundance assessment.

Main Results

Successful preparation and sectioning of Drosophila brain tissues for imaging.
Ensured accurate lipid visualization and analysis through proper sample handling.
Validated methodology enhances the reliability of mass spectrometry imaging data.

Conclusions

This study illustrates the efficacy of MALDI mass spectrometry imaging for lipid analysis in small tissue samples.
It contributes valuable techniques for ongoing biological research into lipids and metabolites.