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Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Article DOI: 10.3791/63968-v • 11:55 min • July 12th, 2022
July 12th, 2022 •
1, 2, 2, 2
1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research

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This protocol describes the formation of supported lipid bilayers and the addition of cytoskeletal filaments and motor proteins to study the dynamics of reconstituted, membrane-tethered cytoskeletal networks using fluorescence microscopy.

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Membrane-tethered Actin Cortices Supported Lipid Bilayers Cytoskeletal Proteins Composite Networks Protocol Cleaning Solution Coplin Jar Sonicator Coverslips Sodium Hydroxide Distilled Water
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