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Immunology and Infection

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Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Article DOI: 10.3791/64032-v • 08:53 min • May 25th, 2022
May 25th, 2022 •
1,2,3, 2,3, 2,3, 2,3, 4, 2,3
1Division of Biology and Biomedical Sciences, Washington University School of Medicine, 2Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, 3Glycobiology Research and Training Center, UCSD, 4Department of Pediatrics, Washington University School of Medicine

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The protocol presents a mouse model of vaginal colonization with anaerobically cultured human vaginal bacteria. We focus on Gardnerella vaginalis, while including suggestions for Prevotella bivia and Fusobacterium nucleatum. This protocol can also be used as a guide for vaginal inoculations and viable recovery of other anaerobically grown bacteria.

Tags

Murine Vaginal Colonization Anaerobically Grown Bacteria Biology Pathophysiology Female Reproductive Tract Aerobic Conditions Anaerobic Bacteria Oxygen Exposure Bacterial Strains Modifications Bacterial Culture Optical Density (OD) Spectrophotometer Inoculum Density Microcentrifuge Tube Anaerobic PBS
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