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Experimentell melanomimmunterapimodell med tumörvaccination med ett hematopoetiskt cytokin

Article DOI: 10.3791/64082-v • 09:15 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1
1La Jolla Institute for Immunology

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Protokollet presenterar en cancerimmunterapimodell med cellbaserad tumörvaccination med Flt3L-uttryckande B16-F10-melanom. Detta protokoll visar procedurerna, inklusive beredning av odlade tumörceller, tumörimplantation, cellbestrålning, mätning av tumörtillväxt, isolering av intratumorala immunceller och flödescytometrianalys.

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Cancerforskning Utgåva 192 Flt3L DC B16-F10 melanom tumörvaccin medfödd immunitet intratumorala immunceller
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