생체 내 마우스 턱밑샘의 혈관 투과성 검출

Overview

This study evaluates the endothelial barrier function of the submandibular gland (SMG) using in vivo imaging techniques. Fluorescent tracers of varying molecular weights were injected into test animal models, demonstrating how molecular permeability can be assessed using two-photon laser scanning microscopy.

Key Study Components

Research Area

• Endothelial barrier function
• Vascular permeability studies
• Fluorescent tracer application

Background

• Importance of tight junctions in endothelial cells
• Non-invasive imaging methods for tissue analysis
• The role of submandibular glands in vascular studies

Methods Used

• In vivo paracellular permeability detection
• Mouse submandibular glands
• Two-photon laser scanning microscopy

Main Results

• Demonstrated varying leakage of fluorescent tracers based on molecular weight
• Identified disruptions in endothelial barrier integrity due to duct ligation
• Validated findings through fluorescence intensity quantification

Conclusions

• The method provides insights into endothelial barrier function in tissues
• Enhances understanding of vascular permeability related to physiological and pathological conditions

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