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Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Article DOI: 10.3791/64274-v • 11:00 min • June 23rd, 2023
June 23rd, 2023 •
1,2, 1
1Children’s Mercy Research Institute, Children’s Mercy Kansas City, 2Department of Pediatrics, University of Missouri-Kansas City School of Medicine

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요약
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Many upregulated genes stimulate tumor cell migration and invasion, leading to poor prognosis. Determining which genes regulate tumor cell migration and invasion is critical. This protocol presents a method for investigating the effects of the increased expression of a gene on the migration and invasion of tumor cells in real time.

Tags

Cancer Research Tumor Cell Migration Invasion Synthetic MRNA Transfection Gene Expression Patterns Tumor Cell Infiltration Metastasis Gene Knockdown Impedance-based Measurement MRNA Vaccines Therapeutic Purposes Gene Overexpression Synthetic MRNA Transfection Stimulation Method Paper Altered Gene Expression
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